Storage And Disposal - 3M Petrifilm 6402 Instrucciones Del Producto

As with any test method, results obtained from use of any 3M Food Safety product do not constitute a guarantee of the quality of the matrices or
processes tested.
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WARRANTIES, INCLUDING BUT NOT LIMITED TO, ANY WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE. If any 3M Food Safety
Product is defective, 3M or its authorized distributor will, at its option, replace or refund the purchase price of the product. These are your exclusive
remedies. You must promptly notify 3M within sixty days of discovery of any suspected defects in a product and return it to 3M. Please call Customer
Service (1-800-328-1671 in the U.S.) or your official 3M Food Safety representative for a Returned Goods Authorization.
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3M WILL NOT BE LIABLE FOR ANY LOSS OR DAMAGES, WHETHER DIRECT, INDIRECT, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES,
INCLUDING BUT NOT LIMITED TO LOST PROFITS. In no event shall 3M's liability under any legal theory exceed the purchase price of the product
alleged to be defective.

STORAGE AND DISPOSAL

Store unopened 3M Petrifilm RCC Plate pouches refrigerated or frozen at temperatures less than or equal to 8ºC (46ºF). Just prior to use, allow
unopened 3M Petrifilm RCC Plate pouches to come to room temperature before opening (20-25°C / <60% RH). Return unused 3M Petrifilm RCC
Plates to pouch. Seal by folding the end of the pouch over and applying adhesive tape. To prevent exposure to moisture, do not refrigerate opened
pouches. Store resealed pouches in a cool dry place for no longer than one month.
It is recommended that resealed pouches of 3M Petrifilm RCC Plates be stored in a freezer (see below) if the laboratory temperature exceeds 25ºC
(77ºF) and/or the laboratory is located in a region where the relative humidity exceeds 50% (with the exception of air-conditioned premises).
To store opened pouches in a freezer, place 3M Petrifilm RCC Plates in a sealable container. To remove frozen 3M Petrifilm RCC Plates for use, open
the container, remove the plates that are needed and immediately return remaining plates to the freezer in the sealed container. 3M Petrifilm RCC
Plates should not be used past their expiration date. The freezer that is used for open pouch storage must not have an automatic defrost cycle as this
would repeatedly expose the 3M Petrifilm RCC Plates to moisture which can damage the plates.
Do not use 3M Petrifilm RCC Plates that show orange or brown discoloration. Expiration date and lot number are noted on each package of 3M
Petrifilm RCC Plates. The lot number is also noted on individual 3M Petrifilm RCC Plates.
W DISPOSAL
After use, 3M Petrifilm RCC Plates may contain microorganisms that may be a potential biohazard. Follow current industry standards for disposal.
INSTRUCTIONS FOR USE
Follow all instructions carefully. Failure to do so may lead to inaccurate results.
Sample Preparation
1. Use appropriate sterile diluents:
Butterfield's phosphate buffer
water. See section,"Specific Instructions for Validated Methods" for specific requirements.
Do not use diluents containing citrate, bisulfite or thiosulfate with 3M Petrifilm RCC Plates; they can inhibit growth. If citrate buffer is
indicated in the standard procedure, substitute with one of the buffers listed above, warmed to 40-45°C (104-113°F).
2. Blend or homogenize sample.
3. For optimal growth and recovery of microorganisms, adjust the pH of the sample suspension to 6.5 - 7.5. For acidic products, adjust the pH with
1N NaOH. For alkaline products, adjust the pH with 1N HCl.
Plating
1. Place the 3M Petrifilm RCC Plate on a flat, level surface (see Figure A).
2. Lift the top film and with the pipette perpendicular dispense 1 mL of sample suspension onto the center of bottom film (see Figure B).
3. Roll the top film down onto the sample to prevent trapping air bubbles (see Figure C).
4. Place the 3M™ Petrifilm™ Spreader with the flat side down on the center of the plate (see Figure D). Press gently on the center of the 3M
Petrifilm Spreader to distribute the sample evenly. Spread the inoculum over the entire 3M Petrifilm RCC Plate growth area before the gel is
formed. Do not slide the spreader across the film.
5. Remove the spreader and leave the plate undisturbed for at least one minute to permit the gel to form.
Incubation
1. Incubate 3M Petrifilm RCC Plates in a horizontal position with the clear side up in stacks of no more than 20 plates. Several incubation times
and temperatures can be used depending on current local reference methods, some of which are listed in the section below titled Specific
Instructions for Validated Methods.
, 0.1% peptone water , peptone salt diluent
6 7
saline solution (0.85-0.90%), bisulfite-free letheen broth, or distilled
6,7
2
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